| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grove, Sabrina A (8)
|
GUCATS |
|
24.01 |
- |
| 2 |
Rana, Samantha S (8)
|
GUTWST |
26.20 |
24.58 |
-1.62 |
| 3 |
Reardon, Madeline E (8)
|
GUTWST |
24.86 |
25.21 |
0.35 |
| 4 |
Cooper, Alanna J (8)
|
GUSST |
23.18 |
25.32 |
2.14 |
| 5 |
McEvoy, Charlee F (8)
|
GUSWAT |
25.72 |
25.36 |
-0.36 |
| 6 |
Medlin, Mia G (7)
|
GUESA |
30.52 |
26.24 |
-4.28 |
| 7 |
Sanchez, Adelle P (8)
|
GUTWST |
25.97 |
26.85 |
0.88 |
| 8 |
Herrera, Iris M (8)
|
GUTWST |
26.35 |
27.36 |
1.01 |
| 9 |
Bajaj, Aryla (7)
|
GUTWST |
29.71 |
28.30 |
-1.41 |
| 10 |
Wiles, Sydney C (7)
|
GUTWST |
27.79 |
28.72 |
0.93 |
| 11 |
Searle, Maya E (8)
|
GUTWST |
|
29.16 |
- |
| 12 |
FitzSullivan, Gabrielle S (7)
|
GUTWST |
30.05 |
29.45 |
-0.60 |
| 13 |
Luscomb, Victoria H (7)
|
GUTWST |
31.23 |
33.61 |
2.38 |
| 14 |
Goss, Layla L (8)
|
GUTWST |
47.47 |
37.38 |
-10.09 |
| 15 |
Graves, Willa M (6)
|
GUTWST |
41.25 |
43.13 |
1.88 |
|
Henry, Paige T (8)
|
GUTWST |
46.29 |
DQ |
- |
|
Messick, Avery M (5)
|
GUTWST |
37.20 |
DQ |
- |
|
Rojas, Allison J (5)
|
GUTWST |
|
DQ |
- |
|
Rosas Magos, Andrea (7)
|
GUTWST |
|
DQ |
- |
|
Bodnar, Ava L (5)
|
GUTWST |
37.88 |
DQ |
- |
|
Bayer, Marie K (7)
|
GUTWST |
43.00 |
DQ |
- |
|
Harrypersad, Nikki (8)
|
GUTWST |
28.93 |
NS |
- |
|
FitzSullivan, Isabella M (7)
|
GUTWST |
32.37 |
NS |
- |
|
Lara, Sophia I (8)
|
GUTWST |
|
NS |
- |
|
Castor, Jordyn A (5)
|
GUTWST |
|
NS |
- |