| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Jordan T (12)
|
GUCFSC |
4:41.65 |
4:43.15 |
1.50 |
| 2 |
Prabhakar, Haasini (12)
|
GUCFSC |
4:45.28 |
4:46.83 |
1.55 |
| 3 |
Ianeva, Monica R (12)
|
GUTWST |
4:48.23 |
4:52.51 |
4.28 |
| 4 |
Phan, Kaitlyn T (12)
|
STNTRO |
4:59.26 |
4:54.75 |
-4.51 |
| 5 |
Dunn, Alexandra L (12)
|
STNTRO |
5:20.00 |
5:09.89 |
-10.11 |
| 6 |
Maciarz, Lola M (11)
|
STNTRO |
5:30.00 |
5:17.58 |
-12.42 |
| 7 |
Domilise, Adeline M (12)
|
GUTWST |
5:39.99 |
5:20.05 |
-19.94 |
| 8 |
Radford, Mackenzie M (12)
|
GUTWST |
5:20.11 |
5:20.32 |
0.21 |
| 9 |
Dillon, Avery C (12)
|
GUKATY |
|
5:22.28 |
- |
| 10 |
Vujosevic, Victoria C (12)
|
STNTRO |
5:30.87 |
5:26.65 |
-4.22 |
| 11 |
Harkin, Norah L (11)
|
STNTRO |
6:24.19 |
5:29.06 |
-55.13 |
| 12 |
Zepeda-Otalora, Alexandra I (12)
|
GUKATY |
5:51.79 |
5:35.83 |
-15.96 |
| 13 |
Johnson, Annemarie M (12)
|
STNTRO |
6:24.19 |
5:45.20 |
-38.99 |
| 14 |
Randall, Cadence E (12)
|
GUESA |
|
5:46.93 |
- |
| 15 |
Stoops, Isabelle J (11)
|
GUKATY |
|
6:08.58 |
- |