| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Evelyn T (6)
|
GULSST |
40.17 |
33.97 |
-6.20 |
| 2 |
McMichael, Quinn C (6)
|
GUTYES |
36.67 |
35.14 |
-1.53 |
| 3 |
Charoenrattanaruk, Alisa (6)
|
GUFCST |
|
38.48 |
- |
| 4 |
Villarreal, Lillianna H (5)
|
GUBTA |
|
38.95 |
- |
| 5 |
Nguyen, Tianna K (6)
|
GUESCA |
44.46 |
40.20 |
-4.26 |
| 6 |
Reese, Adelaide E (6)
|
GUESA |
44.17 |
43.16 |
-1.01 |
| 7 |
Tinajero, Anabella S (5)
|
GUFCST |
49.82 |
44.07 |
-5.75 |
| 8 |
McGee, Averie M (6)
|
GULSST |
47.24 |
44.77 |
-2.47 |
| 9 |
Sisemore, Caroline N (5)
|
GUESA |
|
46.10 |
- |
|
Richards, Ruby P (5)
|
GUBTA |
|
DQ |
- |
|
Vu, Gracelynn N (5)
|
GUFCST |
47.26 |
DQ |
- |
|
Kiyashchenko, Darya (6)
|
GULIFE |
|
DQ |
- |
|
Johnson, Peyton E (6)
|
GUKATY |
|
NS |
- |