| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lucas, Emma J (6)
|
GUVSST |
20.06 |
20.43 |
0.37 |
| 2 |
McGee, Averie M (6)
|
GULSST |
24.53 |
23.96 |
-0.57 |
| 3 |
Nguyen, Tianna K (6)
|
GUESCA |
27.57 |
24.73 |
-2.84 |
| 4 |
Wilson, Piper M (5)
|
GULSST |
25.69 |
24.92 |
-0.77 |
| 5 |
Hines, Sydney A (6)
|
GUVSST |
|
25.23 |
- |
| 6 |
Kiyashchenko, Darya (6)
|
GULIFE |
26.40 |
25.25 |
-1.15 |
| 7 |
Mathew, Samantha L (6)
|
GUFCST |
31.22 |
26.37 |
-4.85 |
| 8 |
Caver, Lauren A (6)
|
GUSPA |
27.05 |
26.51 |
-0.54 |
| 9 |
Atkinson, Juliet R (6)
|
GUBATS |
|
27.29 |
- |
| 10 |
Nguyen, Evelyn T (6)
|
GULSST |
27.83 |
27.34 |
-0.49 |
| 11 |
McMichael, Quinn C (6)
|
GUTYES |
28.84 |
27.60 |
-1.24 |
| 12 |
Bauto, Lia Nicole H (6)
|
GUPEAK |
34.44 |
27.63 |
-6.81 |
| 13 |
Charoenrattanaruk, Alisa (6)
|
GUFCST |
25.64 |
28.10 |
2.46 |
| 14 |
Galvez, Sabine A (6)
|
GUESCA |
27.95 |
29.00 |
1.05 |
| 15 |
Tinajero, Anabella S (5)
|
GUFCST |
30.81 |
29.01 |
-1.80 |
| 16 |
Vu, Gracelynn N (5)
|
GUFCST |
24.94 |
29.20 |
4.26 |
| 17 |
Gasson, Emily P (5)
|
GUVSST |
31.25 |
29.51 |
-1.74 |
| 18 |
Despotovic, Ava W (6)
|
GUPEAK |
33.63 |
29.53 |
-4.10 |
| 19 |
Jehlik, Reilly H (6)
|
GUVSST |
30.57 |
30.16 |
-0.41 |
| 20 |
Villarreal, Lillianna H (5)
|
GUBTA |
30.67 |
31.84 |
1.17 |
| 21 |
Richards, Ruby P (5)
|
GUBTA |
32.97 |
32.06 |
-0.91 |
| 22 |
Carreras, Sophia A (6)
|
GUBTA |
35.60 |
32.91 |
-2.69 |
| 23 |
Reese, Adelaide E (6)
|
GUESA |
31.16 |
33.19 |
2.03 |
| 24 |
Duncan, Maci R (5)
|
GUCATS |
31.95 |
34.19 |
2.24 |
| 25 |
Layne, Beatrice A (6)
|
GUROCC |
|
35.61 |
- |
| 26 |
Sisemore, Caroline N (5)
|
GUESA |
35.67 |
36.20 |
0.53 |
|
Johnson, Peyton E (6)
|
GUKATY |
28.86 |
NS |
- |
|
Putter, Grace I (6)
|
GUFCST |
|
NS |
- |