| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kobukai, Koto (10)
|
NTLAC |
28.16 |
27.56 |
-0.60 |
| 2 |
Nguyen, Ellie T (10)
|
NTLAC |
29.70 |
29.20 |
-0.50 |
| 3 |
Potter, Tinsley G (10)
|
NTLAC |
31.37 |
31.68 |
0.31 |
| 4 |
Pan-Wang, Alina (9)
|
NTLAC |
34.80 |
33.21 |
-1.59 |
| 5 |
Hernandez, Kirsten Sophia M (9)
|
NTLAC |
36.88 |
34.05 |
-2.83 |
| 6 |
Johnson, Darcy E (9)
|
NTLAC |
34.62 |
35.50 |
0.88 |
| 7 |
Dike, Anaya (10)
|
NTLAC |
38.16 |
35.55 |
-2.61 |
| 8 |
Mancuso, Claire A (10)
|
NTLAC |
37.83 |
36.42 |
-1.41 |
| 9 |
Arce, Emma L (10)
|
NTLAC |
36.03 |
36.95 |
0.92 |
| 10 |
Hand, Isabel K (9)
|
NTLAC |
38.72 |
37.51 |
-1.21 |
| 11 |
Shakya, Ruhee N (9)
|
NTLAC |
39.31 |
38.12 |
-1.19 |
| 12 |
Butcher, Abigail J (10)
|
NTLAC |
39.40 |
38.99 |
-0.41 |
| 13 |
Ngo, Amelia S (10)
|
NTLAC |
41.25 |
39.21 |
-2.04 |
| 14 |
Landry, Kaelyn A (9)
|
NTLAC |
44.18 |
41.00 |
-3.18 |
| 15 |
Maddula, Nishika (10)
|
NTLAC |
43.61 |
42.89 |
-0.72 |
| 16 |
Goyal, Ishika (10)
|
NTLAC |
44.21 |
43.69 |
-0.52 |
| 17 |
Lawshe, Lynette L (10)
|
NTLAC |
|
43.99 |
- |
| 18 |
Jendro, Gabrielle P (9)
|
NTLAC |
50.63 |
45.01 |
-5.62 |
| 19 |
Wang, Yipeng (5)
|
NTLAC |
50.67 |
47.63 |
-3.04 |
| 20 |
Sanders, Chloe G (10)
|
NTLAC |
49.93 |
49.98 |
0.05 |
| 21 |
Westerberg, Abigail J (8)
|
NTLAC |
1:08.09 |
52.68 |
-15.41 |
| 22 |
Johnson, Sydney A (6)
|
NTLAC |
48.22 |
52.85 |
4.63 |
| 23 |
O'Brien, Eily S (9)
|
NTLAC |
|
53.17 |
- |
|
Holladay, Hallie A (7)
|
NTLAC |
41.52 |
NS |
- |
|
Southern, Kennedi H (10)
|
NTLAC |
28.96 |
NS |
- |