| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Uliasz, Clair B (12)
|
NTLAC |
28.16 |
27.96 |
-0.20 |
| 2 |
Kruzel, Parker R (11)
|
NTLAC |
29.33 |
28.55 |
-0.78 |
| 3 |
Ross, Callista A (12)
|
NTLAC |
30.07 |
29.22 |
-0.85 |
| 4 |
Sativada, Sai Nandita (12)
|
NTLAC |
29.57 |
29.58 |
0.01 |
| 5 |
Fox, Alexandra T (12)
|
NTLAC |
30.86 |
30.21 |
-0.65 |
| 6 |
Spencer, Macy L (12)
|
NTLAC |
30.70 |
30.43 |
-0.27 |
| 7 |
Schwendinger, Elise W (11)
|
NTLAC |
31.59 |
31.29 |
-0.30 |
| 8 |
Greer, McKennah K (11)
|
NTLAC |
32.17 |
32.27 |
0.10 |
| 9 |
Kraft, Sarah L (11)
|
NTLAC |
33.39 |
32.69 |
-0.70 |
| 10 |
Smith, Arianna R (11)
|
NTLAC |
36.63 |
35.59 |
-1.04 |
|
Schellenger, Nadiyah J (12)
|
NTLAC |
29.32 |
NS |
- |
|
Bibza, Emma E (12)
|
NTNTN |
26.51 |
NS |
- |