| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Andruss, Lily G (10)
|
NTLAC |
5:30.28 |
5:24.23 |
-6.05 |
| 2 |
Guglielmello, Anelle C (10)
|
GUVSST |
5:43.69 |
5:38.83 |
-4.86 |
| 3 |
Corbin, Mackenzie G (10)
|
NTTFA |
5:47.94 |
5:40.69 |
-7.25 |
| 4 |
Cusack, Courtney M (10)
|
NTMTRO |
5:47.22 |
5:47.02 |
-0.20 |
| 5 |
Canales, Kate E (10)
|
STAQSA |
5:50.38 |
5:51.94 |
1.56 |
| 6 |
Poole, Brooklyn M (10)
|
GUAGS |
5:54.47 |
5:52.80 |
-1.67 |
| 7 |
Nguyen, Elise K (10)
|
STNTRO |
5:58.24 |
5:52.82 |
-5.42 |
| 8 |
Horak, Jillian A (10)
|
GUCFSC |
6:00.37 |
5:54.34 |
-6.03 |
| 9 |
Ware, Haley V (9)
|
NTTFA |
6:06.14 |
5:54.56 |
-11.58 |
| 10 |
Beeson, Grier G (10)
|
NTESP |
5:59.92 |
5:59.96 |
0.04 |
| 11 |
Coreas, Maya K (10)
|
GUMAC |
6:04.25 |
6:03.47 |
-0.78 |
| 12 |
Edwards, Simone A (9)
|
NTDM |
6:02.29 |
6:03.63 |
1.34 |
| 13 |
Hale, Saylor Z (9)
|
STNTRO |
6:02.91 |
6:04.82 |
1.91 |
| 14 |
Teeters, Ruby Rose S (10)
|
STNTRO |
6:06.55 |
6:05.66 |
-0.89 |