| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wiles, Sydney C (8)
|
GUTWST |
24.00 |
22.34 |
-1.66 |
| 2 |
FitzSullivan, Gabrielle S (8)
|
GUTWST |
27.23 |
24.36 |
-2.87 |
| 3 |
FitzSullivan, Isabella M (8)
|
GUTWST |
32.37 |
25.42 |
-6.95 |
| 4 |
Rautenbach, Hadley A (6)
|
GUTWST |
34.54 |
29.81 |
-4.73 |
| 5 |
Luscomb, Victoria H (8)
|
GUTWST |
30.80 |
29.89 |
-0.91 |
| 6 |
Bogdanets, Alexis O (8)
|
GUTWST |
33.89 |
30.29 |
-3.60 |
| 7 |
Lucas, Emma J (6)
|
GUVSST |
32.05 |
32.34 |
0.29 |
|
Bunge, Sage L (6)
|
GUTWST |
40.46 |
DQ |
- |
|
Weeks, Clara J (4)
|
GUTWST |
|
DQ |
- |
|
Van Beljon, Amy J (8)
|
GUVSST |
28.82 |
DQ |
- |
|
Seawell, Talley M (8)
|
GUTWST |
|
NS |
- |