| # | Swimmer | Team | Seed | Time | Diff |
| 4 |
Lo, Cynthia B (10)
|
GUDADS |
49.89 |
50.78 |
0.89 |
| 6 |
Belmar, Raegan M (8)
|
GUDADS |
55.63 |
51.79 |
-3.84 |
| 7 |
Olson, Amelia E (9)
|
GUCFSC |
56.57 |
52.16 |
-4.41 |
| 11 |
Cavalcanti, Juliana D (8)
|
GUCFSC |
58.94 |
53.12 |
-5.82 |
| 13 |
Bui, Anjolie (10)
|
GUCFSC |
|
53.75 |
- |
| 14 |
McMahon, Charlotte R (10)
|
GUDADS |
|
54.35 |
- |
| 15 |
Clechenko, Helen P (10)
|
GUDADS |
58.61 |
54.85 |
-3.76 |
| 16 |
Pham, Allyson (8)
|
GUCFSC |
|
55.33 |
- |
| 17 |
Pham, Lana P (9)
|
GUCFSC |
|
55.42 |
- |
| 18 |
Dwyer, Lena E (10)
|
GUDADS |
|
55.47 |
- |
| 19 |
Belmar, Breanna N (8)
|
GUDADS |
57.15 |
55.56 |
-1.59 |
| 20 |
Wente, Brooklyn E (8)
|
GUCFSC |
|
56.16 |
- |
| 28 |
Bhakta, Amiyah M (10)
|
GUCFSC |
1:03.05 |
1:01.27 |
-1.78 |
| 29 |
DeVany, Emily C (10)
|
GUDADS |
1:07.06 |
1:01.68 |
-5.38 |
| 30 |
McCrackin, Kendal G (9)
|
GUCFSC |
|
1:03.35 |
- |
| 31 |
Ramirez, Yaretzi A (9)
|
GUDADS |
|
1:03.96 |
- |
|
Nealon, Renee A (10)
|
GUDADS |
44.89 |
NS |
- |