| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Muse, Naomi E (15)
|
NTTRAC |
|
3:11.02 |
- |
| 2 |
Lemmert, Olivia L (15)
|
NTTRAC |
3:23.42 |
3:13.69 |
-9.73 |
| 3 |
Horne, Jamie A (17)
|
LAYNWL |
3:19.04 |
3:19.93 |
0.89 |
| 4 |
Carberry, Emma C (16)
|
LAYNWL |
3:52.27 |
3:32.41 |
-19.86 |
| 5 |
Courtney, Natalie L (15)
|
GUAMBU |
3:46.26 |
3:32.75 |
-13.51 |
| 6 |
Stout, Taylor G (16)
|
NTMTRO |
|
3:35.69 |
- |
| 7 |
Haase, Liberty E (15)
|
LAYNWL |
3:31.27 |
3:40.18 |
8.91 |
| 8 |
Vahovius, Mary-Catherine (17)
|
NTMTRO |
|
4:14.37 |
- |
| 9 |
Romanski, Isabella G (15)
|
LAYNWL |
|
4:29.45 |
- |
|
Jackson, Abigail E (16)
|
LAYNWL |
3:24.75 |
NS |
- |