| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Kaden J (14)
|
GUSPA |
56.78 |
54.46 |
-2.32 |
| 2 |
Salazar, Santiago (13)
|
GUPFL |
56.59 |
55.58 |
-1.01 |
| 3 |
DeMoss, Jackson E (14)
|
GUTYES |
59.55 |
56.14 |
-3.41 |
| 4 |
Brito, Thomas A (14)
|
GUPFL |
|
56.56 |
- |
| 5 |
Farahbod, Benjamin C (13)
|
GUDADS |
57.04 |
56.62 |
-0.42 |
| 6 |
Pabon, Sebastian J (13)
|
GUTEST |
58.73 |
58.21 |
-0.52 |
| 7 |
Moss, Nathan E (13)
|
GUDADS |
58.25 |
58.26 |
0.01 |
| 8 |
Alarcon, Elias (13)
|
GUSWAT |
58.93 |
58.64 |
-0.29 |
| 9 |
Salgado, Dillan M (13)
|
GUTYES |
59.15 |
58.65 |
-0.50 |
| 10 |
Gupta, Rutva (14)
|
GUECS |
56.91 |
58.91 |
2.00 |
| 11 |
Brenton, Cameron L (13)
|
GUDADS |
58.20 |
58.98 |
0.78 |
| 12 |
Avila, Victor M (14)
|
GUHCAP |
1:00.38 |
59.19 |
-1.19 |
| 13 |
Kellermayer, Abris B (14)
|
GUTEST |
1:01.57 |
59.21 |
-2.36 |
| 14 |
Lee, Maxton T (13)
|
GUDADS |
1:02.37 |
59.31 |
-3.06 |
| 15 |
Belloso, Alan S (14)
|
GUDADS |
59.84 |
59.34 |
-0.50 |
| 16 |
Hackney, James A (13)
|
GUDADS |
59.59 |
59.37 |
-0.22 |
| 16 |
Brandt, Luke J (14)
|
GUTEST |
1:01.80 |
59.37 |
-2.43 |
| 18 |
Bautista, Jared I (14)
|
GUHCAP |
1:02.00 |
1:00.35 |
-1.65 |
| 19 |
Dionne, Grant T (14)
|
GUTEST |
1:01.48 |
1:00.43 |
-1.05 |
| 20 |
Bobbili, Karthik K (13)
|
GUECS |
1:12.52 |
1:00.65 |
-11.87 |
| 21 |
Larsson, Mikael (13)
|
GUECS |
1:04.26 |
1:00.77 |
-3.49 |
| 22 |
Gadin, Christophe L (13)
|
GUDADS |
|
1:00.91 |
- |
| 23 |
Miranda, Maccio A (13)
|
GUTEST |
1:00.40 |
1:01.78 |
1.38 |
| 24 |
Byrd, Jalen A (13)
|
GUSWAT |
1:01.11 |
1:02.21 |
1.10 |
| 25 |
Hemmer, Julian A (13)
|
GUDADS |
1:03.36 |
1:02.37 |
-0.99 |
| 26 |
Herrmann, Harrison R (14)
|
GUTEST |
1:03.91 |
1:02.98 |
-0.93 |
| 27 |
Dillett, Anthony M (13)
|
GUSPA |
1:03.74 |
1:03.51 |
-0.23 |
| 28 |
Oliver, Noah G (14)
|
GUHCAP |
|
1:03.80 |
- |
| 29 |
Anglin, Brody A (13)
|
GUTYES |
|
1:04.29 |
- |
| 30 |
Ettinger, Avi J (13)
|
GUSPA |
|
1:05.03 |
- |
| 31 |
Platon, Iancu P (13)
|
GUECS |
1:07.10 |
1:06.07 |
-1.03 |
| 32 |
Robertson, Cadence C (13)
|
GUTEST |
1:10.20 |
1:07.73 |
-2.47 |
| 33 |
Sukumaran, Akshay H (13)
|
GUPFL |
|
1:09.91 |
- |
| 34 |
Reeves, Parker C (14)
|
GUDADS |
|
1:10.13 |
- |
| 35 |
Frank, Sam J (13)
|
GUDADS |
1:14.60 |
1:10.96 |
-3.64 |
| 36 |
Bellew, Joseph R (13)
|
GUSWAT |
1:15.32 |
1:16.65 |
1.33 |
| 37 |
Nicksic, Eli D (13)
|
GUPFL |
1:26.55 |
1:18.67 |
-7.88 |
|
Conticello, Richard A (13)
|
GUDADS |
1:10.67 |
NS |
- |
|
Guthrie, Christopher R (13)
|
GUDADS |
|
NS |
- |