| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Evie M (10)
|
GUSWAT |
2:54.70 |
2:51.52 |
-3.18 |
| 2 |
Pham, Kaitlyn C (10)
|
GUTEST |
3:00.41 |
2:51.84 |
-8.57 |
| 3 |
Chin, Yilian S (10)
|
GUKATY |
3:09.05 |
2:53.95 |
-15.10 |
| 4 |
Lajaunie, Sarah C (9)
|
GUFCST |
3:00.20 |
2:57.91 |
-2.29 |
| 5 |
St John, Laura E (10)
|
GUSHAC |
3:10.66 |
2:58.17 |
-12.49 |
| 6 |
Williford, Elise F (9)
|
GUFCST |
3:04.55 |
2:58.47 |
-6.08 |
| 7 |
Morgan, Jayde K (10)
|
GUSHAC |
3:10.68 |
2:58.79 |
-11.89 |
| 8 |
Zhao, Serena (9)
|
GU300 |
3:05.00 |
3:03.00 |
-2.00 |
| 9 |
Turan, Ecem Y (9)
|
GUESCA |
3:13.64 |
3:03.71 |
-9.93 |
| 10 |
Wernet, Brooke A (10)
|
GUKATY |
3:11.05 |
3:04.17 |
-6.88 |
| 11 |
Elgar, Kate Jasmine E (10)
|
GUSHAC |
3:03.73 |
3:04.44 |
0.71 |
| 12 |
Boettcher, Kyleigh M (10)
|
GUSWAT |
3:09.73 |
3:04.45 |
-5.28 |
| 13 |
Hayes, Charli D (8)
|
GUFCST |
3:07.90 |
3:04.69 |
-3.21 |
| 14 |
Fu, Sophia Y (8)
|
GUKATY |
3:18.35 |
3:04.73 |
-13.62 |
| 15 |
Roberts, Kaylee M (9)
|
GUPACE |
|
3:04.92 |
- |
| 16 |
McGonagle, Brielle (9)
|
GUSSS |
3:14.40 |
3:04.95 |
-9.45 |
| 17 |
Carey, Mia A (9)
|
GUESCA |
2:55.11 |
3:05.14 |
10.03 |
| 18 |
Luedde, Katie A (10)
|
GUSSS |
3:13.77 |
3:07.49 |
-6.28 |
| 19 |
Luca, Ecaterina (10)
|
GUECS |
3:03.37 |
3:07.98 |
4.61 |
| 20 |
Herbst, Grace E (10)
|
GUSHAC |
3:17.66 |
3:08.20 |
-9.46 |
| 21 |
Horton, Julianne R (10)
|
GUKATY |
3:14.33 |
3:09.12 |
-5.21 |
| 22 |
Valchar, Addley P (7)
|
GUSHAC |
3:25.09 |
3:12.50 |
-12.59 |
| 23 |
LeBlanc, Katelyn A (10)
|
GUPEAK |
3:15.59 |
3:14.39 |
-1.20 |
| 24 |
Hulbert, Arianna R (9)
|
GUFCST |
3:16.15 |
3:19.00 |
2.85 |
| 25 |
Schmidt, Laney S (10)
|
GUFCST |
3:24.47 |
3:20.60 |
-3.87 |
| 26 |
Usagani, Katherine D (9)
|
GUSPA |
3:26.08 |
3:28.98 |
2.90 |
| 27 |
Morvant, Renee J (8)
|
GUFCST |
3:26.82 |
3:30.84 |
4.02 |
| 28 |
Jones, Madison M (9)
|
GUSPA |
3:32.15 |
3:34.82 |
2.67 |