| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stapleton, Tallis M (7)
|
GUSHAC |
17.72 |
17.41 |
-0.31 |
| 2 |
Lindberg, Ainsley E (7)
|
GUBTA |
18.57 |
19.30 |
0.73 |
| 3 |
Tisdall, Reese H (7)
|
GURICE |
22.66 |
20.46 |
-2.20 |
| 4 |
Casperson, Cara E (7)
|
GUBTA |
18.55 |
21.88 |
3.33 |
| 5 |
Riffel, Gemma A (7)
|
GUSHAC |
22.82 |
23.19 |
0.37 |
| 6 |
Martinez, Azul (7)
|
GUHCAP |
21.32 |
23.80 |
2.48 |
| 7 |
Partin, Fiona M (7)
|
GUUN-S |
39.59 |
25.01 |
-14.58 |
| 8 |
Fontenot, Kalyssa M (7)
|
GUSHAC |
|
25.92 |
- |
| 9 |
Zhao, Jordana L (7)
|
GUPEAK |
30.90 |
28.99 |
-1.91 |
| 10 |
Villarreal, Addyson G (7)
|
GUBTA |
28.40 |
29.66 |
1.26 |
| 11 |
Foertsch, Katherine E (7)
|
GURICE |
|
38.43 |
- |
| 12 |
McManus, Ashlyn M (7)
|
GUPEAK |
|
40.08 |
- |
|
Vinson, Kaeden G (7)
|
GUPEAK |
49.06 |
DQ |
- |