| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Siemer, Spencer F (17)
|
GULSST |
22.51 |
23.30 |
0.79 |
| 2 |
Barbosa, Sebastian (17)
|
GUPFL |
|
23.77 |
- |
| 3 |
Tankovich, Adrian (15)
|
GUPFL |
24.47 |
24.93 |
0.46 |
| 4 |
Kondapalli, Sriharsha (15)
|
GUUN-T |
25.03 |
24.98 |
-0.05 |
| 5 |
Gonzalez, Alexander I (16)
|
GUKATY |
25.38 |
26.12 |
0.74 |
| 5 |
Malak, Mazen D (16)
|
GUTEST |
26.73 |
26.12 |
-0.61 |
| 7 |
Riede Noriega, Andre D (15)
|
GUKATY |
26.52 |
26.84 |
0.32 |
| 8 |
Bender, Rhys C (17)
|
GUSCAT |
27.37 |
27.84 |
0.47 |
| 9 |
Fabian, Matthew E (17)
|
GUHUNT |
28.17 |
28.12 |
-0.05 |
| 10 |
Bishop, Hunter J (15)
|
GUECS |
28.50 |
28.73 |
0.23 |
| 11 |
McDaniel, Austin J (17)
|
GULIFE |
32.61 |
30.99 |
-1.62 |