| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Diaz, Mariana (13)
|
GUPFL |
2:39.97 |
2:42.54 |
2.57 |
| 2 |
Koenst, Katelyn M (13)
|
GUSCAT |
2:41.98 |
2:45.25 |
3.27 |
| 3 |
Musselman, Jordan M (13)
|
GUKATY |
|
2:54.14 |
- |
| 4 |
Mills, Kendall G (14)
|
GUSWIM |
2:56.05 |
2:54.40 |
-1.65 |
| 5 |
Katz, Madeline G (13)
|
GUTEST |
3:31.23 |
2:55.83 |
-35.40 |
| 6 |
Moonsammy, Vanessa C (14)
|
GUKATY |
2:51.40 |
2:57.27 |
5.87 |
| 7 |
Freeman, Abigail J (14)
|
GUTYES |
3:01.58 |
3:02.28 |
0.70 |
| 8 |
Dumitrascu, Valeria (13)
|
GUKATY |
|
3:02.57 |
- |
| 9 |
Osode, Ngozi F (14)
|
GUKATY |
3:06.51 |
3:05.50 |
-1.01 |
| 10 |
Veit, Taylor G (14)
|
GUTYES |
3:02.49 |
3:09.79 |
7.30 |
| 11 |
Reuber, Allison S (13)
|
GUKATY |
3:11.64 |
3:11.35 |
-0.29 |
| 12 |
Derby, Sophie J (13)
|
GUPFL |
|
3:16.25 |
- |
| 13 |
Kamma, Bhavika (13)
|
GUKATY |
|
3:21.28 |
- |
| 14 |
Brosch, Morgan O (13)
|
GUPFL |
3:21.56 |
3:22.64 |
1.08 |
| 15 |
Burns, Gracie I (13)
|
GUPFL |
3:47.14 |
3:38.27 |
-8.87 |
| 16 |
Malachowski, Ashlyn D (13)
|
GUKATY |
|
3:38.95 |
- |
|
Landin-Seneriz, Adriana (14)
|
GUKATY |
3:14.16 |
NS |
- |