| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Siemer, Spencer F (17)
|
GULSST |
49.71 |
51.16 |
1.45 |
| 2 |
Kennedy, Kevan D (17)
|
GUPFL |
49.49 |
51.69 |
2.20 |
| 3 |
Barbosa, Sebastian (17)
|
GUPFL |
52.52 |
52.76 |
0.24 |
| 4 |
Mann, Colby P (16)
|
GUPFL |
53.43 |
53.96 |
0.53 |
| 5 |
Tankovich, Adrian (15)
|
GUPFL |
54.62 |
55.52 |
0.90 |
| 6 |
Kondapalli, Sriharsha (15)
|
GUUN-T |
56.40 |
55.77 |
-0.63 |
| 7 |
Malak, Mazen D (16)
|
GUTEST |
57.90 |
56.07 |
-1.83 |
| 8 |
Ulrich, Cooper E (15)
|
GUTTST |
59.50 |
58.68 |
-0.82 |
| 9 |
Bender, Rhys C (17)
|
GUSCAT |
58.61 |
59.27 |
0.66 |
| 10 |
Bishop, Hunter J (15)
|
GUECS |
1:01.80 |
1:01.07 |
-0.73 |
| 11 |
Fabian, Matthew E (17)
|
GUHUNT |
1:03.73 |
1:02.34 |
-1.39 |
| 12 |
Gurfinkel, Armando (15)
|
GUPFL |
1:09.75 |
1:05.84 |
-3.91 |
| 13 |
McDaniel, Austin J (17)
|
GULIFE |
1:11.59 |
1:09.96 |
-1.63 |
|
Imbern, Ezequiel (16)
|
GUTYES |
52.94 |
NS |
- |
|
Wardinsky, William B (16)
|
GUKATY |
1:00.45 |
NS |
- |
|
Mujahed, Youssof E (15)
|
GUKATY |
52.97 |
NS |
- |
|
Gonzalez, Alexander I (16)
|
GUKATY |
53.25 |
NS |
- |