| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rasmussen, Katherine (11)
|
GUAGS |
2:37.81 |
2:25.78 |
-12.03 |
| 2 |
McDonald, Samantha R (12)
|
GUTASC |
2:35.04 |
2:36.66 |
1.62 |
| 3 |
Smith, Hailey M (11)
|
GUSSAN |
2:56.84 |
2:36.83 |
-20.01 |
| 4 |
Fulton, Elsa A (11)
|
GUTWST |
2:50.24 |
2:42.46 |
-7.78 |
| 5 |
Vance, Elisabeth L (11)
|
GUSST |
2:51.77 |
2:44.79 |
-6.98 |
| 6 |
Buenemann, Jayden (12)
|
GUAGS |
2:51.53 |
2:49.02 |
-2.51 |
| 7 |
Salas Sanchez, Sofia (12)
|
GUSST |
|
2:51.45 |
- |
| 8 |
Hall, Kaley B (12)
|
GUTWST |
2:53.10 |
2:55.77 |
2.67 |
| 9 |
Crane, Sydney D (12)
|
GUMAC |
3:00.19 |
2:56.82 |
-3.37 |
| 10 |
Laubsch, Melania M (12)
|
GUMAC |
2:50.26 |
2:56.91 |
6.65 |
| 11 |
Bearden, Kennedy J (11)
|
GUSSAN |
3:14.96 |
2:58.30 |
-16.66 |
| 12 |
Salas Sanchez, Camila (12)
|
GUSST |
|
3:01.23 |
- |
| 13 |
Holwerda, Ava S (11)
|
GUTWST |
3:09.73 |
3:02.19 |
-7.54 |
| 14 |
Geiger, Kiera M (12)
|
GUTWST |
3:14.02 |
3:12.98 |
-1.04 |
| 15 |
Gonzalez, Skylar S (11)
|
GUTWST |
|
3:13.26 |
- |
| 16 |
Song, Selina J (12)
|
GUTWST |
|
3:15.32 |
- |
| 17 |
Wang, Jessica (11)
|
GUTWST |
|
3:17.97 |
- |
|
McLeroy, Jessica H (12)
|
GUSSAN |
|
NS |
- |
|
Cox, Maeve M (12)
|
GUTWST |
|
NS |
- |
|
Galinsky, Summer L (11)
|
GUTWST |
|
NS |
- |