| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Otwell, Karson D (9)
|
GUSSAN |
2:38.31 |
2:38.00 |
-0.31 |
| 2 |
Goulait, Joseph G (10)
|
GUMARC |
2:51.64 |
2:40.26 |
-11.38 |
| 3 |
Singh, Sahil R (9)
|
GUTWST |
2:41.70 |
2:40.75 |
-0.95 |
| 4 |
Loeffel, William M (10)
|
GUSSAN |
2:39.50 |
2:42.26 |
2.76 |
| 5 |
West, Hugo A (10)
|
GUAGS |
2:47.17 |
2:43.82 |
-3.35 |
| 6 |
Kwok, Jaden J (10)
|
GUAGS |
2:51.04 |
2:45.13 |
-5.91 |
| 7 |
Crenshaw, Holland J (10)
|
GUSSAN |
2:51.08 |
2:45.63 |
-5.45 |
| 8 |
Simons, Maximus A (9)
|
GUTWST |
|
2:49.45 |
- |
| 9 |
Kornreich, Colin J (10)
|
GUTWST |
2:47.41 |
2:50.94 |
3.53 |
| 10 |
Paas, Kenny (10)
|
GUMAC |
3:05.49 |
2:51.21 |
-14.28 |
| 11 |
Marchand, Landon R (10)
|
GUSSAN |
3:08.58 |
2:51.61 |
-16.97 |
| 12 |
Chalmers, Tytus E (10)
|
GUAGS |
2:56.52 |
2:53.20 |
-3.32 |
| 13 |
Yang, Edward (9)
|
GUAGS |
3:04.06 |
2:54.81 |
-9.25 |
| 14 |
Li, Patrick (9)
|
GUAGS |
2:54.14 |
2:56.06 |
1.92 |
| 15 |
Meneses, Nicholas D (9)
|
GUTWST |
|
2:58.21 |
- |
| 16 |
Taylor, Jacob C (9)
|
GUSSAN |
|
3:01.29 |
- |
| 17 |
Chen, Kai Z (9)
|
GUTWST |
3:03.95 |
3:03.58 |
-0.37 |
| 18 |
Beedle, Eichten A (10)
|
GUTWST |
3:04.07 |
3:04.34 |
0.27 |
| 19 |
Petersen, Vincent R (10)
|
GUSSAN |
|
3:04.75 |
- |
| 20 |
Espinosa-Agrait, Esteban A (9)
|
GUTWST |
3:06.73 |
3:08.31 |
1.58 |
| 21 |
Graves, Maximilian W (9)
|
GUTWST |
|
3:08.36 |
- |
| 22 |
Davis, Logan (10)
|
GUEPRC |
|
3:08.43 |
- |
| 23 |
Niewenhous, William H (9)
|
GUTWST |
|
3:10.10 |
- |
| 24 |
Heinsohn, Connor Z (9)
|
GUSSAN |
|
3:20.11 |
- |
| 25 |
Alarcon, Samuel D (10)
|
GUSSAN |
|
3:29.01 |
- |
| 26 |
Gonzalez, Alejandro (9)
|
GUMAC |
4:00.73 |
3:32.81 |
-27.92 |
| 27 |
Nixon, Ethan J (9)
|
GUSSAN |
|
3:46.36 |
- |
|
Koo, Bailey (9)
|
GUAGS |
|
NS |
- |
|
Haenel, Carl G (10)
|
GUMAC |
|
NS |
- |
|
Chen, Thomas (9)
|
GUAGS |
|
NS |
- |