| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nordmann, Lillie G (17)
|
GUMAC |
22.89 |
23.82 |
0.93 |
| 2 |
Staffeldt, ValerieAnne (17)
|
GUUN-M |
23.01 |
24.96 |
1.95 |
| 3 |
Marsh, Noelle L (16)
|
GUMAC |
23.68 |
25.40 |
1.72 |
| 4 |
Daspit, Jolie E (18)
|
GUMAC |
23.48 |
25.64 |
2.16 |
| 5 |
Barrientos, Isabella M (16)
|
GUMAC |
|
26.05 |
- |
| 6 |
Kruchten, Madeline C (17)
|
GUMAC |
24.82 |
26.16 |
1.34 |
| 7 |
Busch, Olivia M (15)
|
GUMAC |
26.84 |
26.76 |
-0.08 |
| 8 |
Ritch, Jane H (17)
|
GUMAC |
25.57 |
27.20 |
1.63 |
| 9 |
Smith, Susannah E (15)
|
GUAGS |
25.51 |
27.48 |
1.97 |
| 10 |
Heitzmann, Presley V (16)
|
GUMAC |
26.91 |
27.99 |
1.08 |
| 11 |
Barry, Elizabeth M (16)
|
GUNEHA |
29.39 |
28.01 |
-1.38 |
| 12 |
Dean, Shelby E (16)
|
GUNEHA |
|
28.26 |
- |
| 13 |
Monroe, Libby (15)
|
GUMAC |
26.99 |
28.28 |
1.29 |
| 14 |
Draeger, Isabelle E (15)
|
GUMAC |
28.93 |
28.80 |
-0.13 |
| 15 |
Jones, Raven A (16)
|
GUMAC |
28.34 |
29.04 |
0.70 |
| 16 |
Richmond, Carolyn R (15)
|
GUMAC |
28.11 |
29.26 |
1.15 |
| 17 |
Riddle, Allia M (16)
|
GUAGS |
30.48 |
30.52 |
0.04 |
| 18 |
Loy, Braelin P (15)
|
GUMAC |
|
30.91 |
- |
| 19 |
Johnson, Averi R (16)
|
GUAGS |
33.08 |
34.49 |
1.41 |
|
Fronczak, Katherine D (17)
|
GUMAC |
|
NS |
- |