| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pan-Wang, Evan (11)
|
NTLAC |
28.59 |
28.06 |
-0.53 |
| 2 |
Roybal, Hudson L (12)
|
NTLAC |
28.93 |
29.11 |
0.18 |
| 3 |
Arangalla, Ramika A (12)
|
NTLAC |
29.54 |
29.24 |
-0.30 |
| 4 |
Maunus, Luke A (12)
|
NTLAC |
29.21 |
29.44 |
0.23 |
| 5 |
Heldenbrand, Dylan F (12)
|
NTNTN |
30.01 |
29.68 |
-0.33 |
| 6 |
Agnihotri, Abhinav A (11)
|
NTLAC |
31.05 |
29.98 |
-1.07 |
| 7 |
Ruhl, Jonah W (11)
|
NTATAC |
29.98 |
30.29 |
0.31 |
| 8 |
Chiang, Tyler B (12)
|
NTLAC |
31.49 |
30.58 |
-0.91 |
| 9 |
Beck, Jackson E (12)
|
NTLAC |
30.13 |
30.63 |
0.50 |
| 10 |
Bolledula, Aditya R (11)
|
NTTFA |
31.01 |
31.20 |
0.19 |
| 11 |
Geheb, Oliver M (12)
|
NTCOPS |
31.60 |
31.78 |
0.18 |
| 12 |
Shih, Theodore K (12)
|
NTLAC |
34.23 |
32.18 |
-2.05 |
| 13 |
Wong, Alexander J (12)
|
NTLAC |
33.58 |
33.30 |
-0.28 |