| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cochran, Luke R (7)
|
GUDADS |
|
23.18 |
- |
| 2 |
Hirshey, Noah A (7)
|
GUCATS |
24.39 |
23.59 |
-0.80 |
| 3 |
Parker, Maximilian G (7)
|
GUECS |
24.53 |
24.05 |
-0.48 |
| 4 |
Lo, Alphonse R (7)
|
GUDADS |
23.85 |
24.21 |
0.36 |
| 5 |
Smith, Preston R (7)
|
GUDADS |
23.29 |
24.57 |
1.28 |
| 6 |
King, Joseph J (7)
|
GUPACK |
22.56 |
24.64 |
2.08 |
| 7 |
Chong, Kellan M (7)
|
GUPACK |
26.84 |
24.98 |
-1.86 |
| 8 |
Van Spankeren, Boma A (7)
|
GUECS |
25.66 |
26.71 |
1.05 |
| 9 |
Vasyankin, Vladimir A (7)
|
GUDADS |
30.50 |
27.65 |
-2.85 |
| 10 |
Poteet, Nikolai J (7)
|
GUECS |
32.21 |
27.88 |
-4.33 |
| 11 |
Kirk, Ethan D (7)
|
GUDADS |
|
32.70 |
- |