| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Aghdasi, Radin (7)
|
GU300 |
49.53 |
45.99 |
-3.54 |
| 2 |
Hirshey, Noah A (7)
|
GUCATS |
1:08.50 |
52.32 |
-16.18 |
| 3 |
Smith, Preston R (7)
|
GUDADS |
53.30 |
52.37 |
-0.93 |
| 4 |
Luswata, Caleb N (7)
|
GUPACK |
52.73 |
52.71 |
-0.02 |
| 5 |
King, Joseph J (7)
|
GUPACK |
53.22 |
53.64 |
0.42 |
| 6 |
Parker, Maximilian G (7)
|
GUECS |
54.09 |
56.11 |
2.02 |
| 7 |
Vasyankin, Vladimir A (7)
|
GUDADS |
1:03.70 |
1:02.67 |
-1.03 |
| 8 |
Shah, Michael S (7)
|
GUPACK |
1:01.69 |
1:03.55 |
1.86 |
| 9 |
Poteet, Nikolai J (7)
|
GUECS |
1:05.42 |
1:04.10 |
-1.32 |
| 10 |
Kirk, Ethan D (7)
|
GUDADS |
1:08.96 |
1:12.61 |
3.65 |
|
Cochran, Luke R (7)
|
GUDADS |
|
DQ |
- |
|
Van Spankeren, Boma A (7)
|
GUECS |
1:00.58 |
DQ |
- |