| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Otwell, Jaden N (8)
|
GUSSAN |
34.28 |
34.27 |
-0.01 |
| 2 |
Yang, Chloe Y (8)
|
GUSSAN |
40.24 |
40.08 |
-0.16 |
| 3 |
Ehlers, Naomi G (8)
|
GUOFSC |
47.74 |
40.96 |
-6.78 |
| 4 |
Casperson, Cara E (8)
|
GUBTA |
46.43 |
42.64 |
-3.79 |
| 5 |
Herbel, Camille C (8)
|
GUSSAN |
46.99 |
43.63 |
-3.36 |
| 6 |
Mehra, Mehak S (8)
|
GUOFSC |
53.77 |
43.95 |
-9.82 |
| 7 |
Herbel, Carissa M (8)
|
GUSSAN |
51.83 |
46.11 |
-5.72 |
| 8 |
Shanor, Kaytin J (8)
|
GUSSAN |
45.50 |
46.18 |
0.68 |
| 9 |
Villarreal, Addyson G (8)
|
GUBTA |
53.20 |
46.39 |
-6.81 |
| 10 |
Kitu, Hannah G (8)
|
GUSCAT |
54.20 |
46.63 |
-7.57 |
| 11 |
Dulworth, Ella M (8)
|
GUSSAN |
52.21 |
47.73 |
-4.48 |
| 12 |
Hadley, Kaitlyn N (8)
|
GUSSAN |
50.86 |
50.20 |
-0.66 |
| 13 |
Matthews, Charlotte G (8)
|
GUSCAT |
1:04.84 |
58.28 |
-6.56 |
| 14 |
Winslow, Ava M (8)
|
GUBTA |
|
1:06.44 |
- |
|
Chandrahasan, Eshana A (8)
|
GUOFSC |
|
NS |
- |