| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Qiu, Selina Y (12)
|
GUSHAC |
27.65 |
26.17 |
-1.48 |
| 2 |
Valchar, Ava G (11)
|
GUSHAC |
27.58 |
27.48 |
-0.10 |
| 3 |
Cardenas, Isabella K (11)
|
GUSHAC |
28.45 |
28.00 |
-0.45 |
| 4 |
Riffel, Gianna A (12)
|
GUSHAC |
29.09 |
28.45 |
-0.64 |
| 5 |
Pham, Elizabeth A (12)
|
GUESCA |
29.48 |
28.58 |
-0.90 |
| 6 |
Ford, Emily G (12)
|
GUUN-S |
29.13 |
28.88 |
-0.25 |
| 7 |
Rosanes, Hannah D (12)
|
GUSHAC |
29.28 |
29.11 |
-0.17 |
| 8 |
Bright, Kaydyn N (11)
|
GUSHAC |
29.85 |
29.16 |
-0.69 |
| 9 |
Chen, Michelle W (11)
|
GUSHAC |
30.59 |
29.69 |
-0.90 |
| 10 |
Ly, Yvonne K (11)
|
GUESCA |
|
29.77 |
- |
| 11 |
Baker, Brooke C (12)
|
GUESCA |
30.73 |
30.32 |
-0.41 |
| 12 |
Klun, Marissa C (11)
|
GUAQUA |
30.76 |
31.69 |
0.93 |
| 13 |
Kong, Cynthia R (12)
|
GUCOOG |
34.13 |
31.71 |
-2.42 |
| 14 |
Asthagiri, Maithreyi D (11)
|
GUCOOG |
32.94 |
32.03 |
-0.91 |
| 15 |
Astramskas, Ava L (11)
|
GUSWAT |
34.18 |
33.41 |
-0.77 |
| 16 |
Inswang, Samantha N (11)
|
GUSHAC |
35.45 |
33.53 |
-1.92 |
| 17 |
Lorber, Ava A (11)
|
GUESCA |
33.12 |
33.61 |
0.49 |
| 18 |
Diaz-Oviedo, Andrea I (12)
|
GUHCAP |
33.58 |
33.97 |
0.39 |
| 19 |
Woodby, Addison N (11)
|
GUAQUA |
34.35 |
34.32 |
-0.03 |
| 20 |
Arya, Ada D (11)
|
GUCOOG |
37.44 |
34.72 |
-2.72 |
| 21 |
Garcia, Elizabeth A (11)
|
GUHCAP |
36.50 |
35.62 |
-0.88 |
| 22 |
Enriquez, Alexa M (12)
|
GUHCAP |
35.43 |
35.68 |
0.25 |
| 23 |
Lau, Christina Y (11)
|
GUSHAC |
39.48 |
35.73 |
-3.75 |
| 24 |
Massey, Jubilee G (11)
|
GUHCAP |
|
35.98 |
- |
| 25 |
Garcia, Leslie F (12)
|
GUAQUA |
34.51 |
36.17 |
1.66 |
| 26 |
Sinha, Revati A (11)
|
GUCOOG |
39.30 |
36.31 |
-2.99 |
| 27 |
Castro, Miaangelina (11)
|
GUHCAP |
39.80 |
37.89 |
-1.91 |
| 28 |
Pooya, Artemiss A (11)
|
GUECS |
|
41.96 |
- |
|
Lorber, Lyla G (11)
|
GUESCA |
35.13 |
NS |
- |
|
Moya, Megan L (12)
|
GUSHAC |
|
NS |
- |
|
Du, Aien (11)
|
GU300 |
33.73 |
NS |
- |