| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Qiu, Selina Y (12)
|
GUSHAC |
34.01 |
34.17 |
0.16 |
| 2 |
Tremaine, Malea T (12)
|
GUESCA |
33.90 |
34.95 |
1.05 |
| 3 |
Riffel, Gianna A (12)
|
GUSHAC |
36.11 |
35.47 |
-0.64 |
| 4 |
Ford, Emily G (12)
|
GUUN-S |
36.81 |
35.75 |
-1.06 |
| 5 |
Pham, Elizabeth A (12)
|
GUESCA |
38.54 |
37.45 |
-1.09 |
| 6 |
Ly, Yvonne K (11)
|
GUESCA |
|
37.58 |
- |
| 7 |
Rosanes, Hannah D (12)
|
GUSHAC |
37.77 |
37.61 |
-0.16 |
| 8 |
Bright, Kaydyn N (11)
|
GUSHAC |
37.88 |
37.84 |
-0.04 |
| 9 |
Astramskas, Ava L (11)
|
GUSWAT |
41.69 |
40.01 |
-1.68 |
| 10 |
Chen, Michelle W (11)
|
GUSHAC |
44.17 |
40.14 |
-4.03 |
| 11 |
Inswang, Samantha N (11)
|
GUSHAC |
45.10 |
41.85 |
-3.25 |
| 12 |
Shen, Amanda H (12)
|
GUSWAT |
42.09 |
42.01 |
-0.08 |
| 13 |
Delasbour, Tatyana J (11)
|
GUHCAP |
|
42.08 |
- |
| 14 |
Baker, Brooke C (12)
|
GUESCA |
39.80 |
42.18 |
2.38 |
| 15 |
Du, Aien (11)
|
GU300 |
47.27 |
42.75 |
-4.52 |
| 16 |
Lopez-Alfaro, Sofia C (12)
|
GUAQUA |
48.03 |
43.49 |
-4.54 |
| 17 |
Lau, Christina Y (11)
|
GUSHAC |
45.82 |
43.54 |
-2.28 |
| 18 |
Lorber, Ava A (11)
|
GUESCA |
47.36 |
44.99 |
-2.37 |
| 19 |
Gow, Alexis K (11)
|
GUSWAT |
49.08 |
46.45 |
-2.63 |
| 20 |
Arya, Ada D (11)
|
GUCOOG |
46.49 |
46.95 |
0.46 |
| 21 |
Hemstra, Marrit L (11)
|
GUSWAT |
55.64 |
47.07 |
-8.57 |
| 22 |
Enriquez, Alexa M (12)
|
GUHCAP |
49.29 |
48.77 |
-0.52 |
| 23 |
Sinha, Revati A (11)
|
GUCOOG |
48.25 |
50.10 |
1.85 |
| 24 |
Garcia, Leslie F (12)
|
GUAQUA |
50.85 |
51.80 |
0.95 |
| 25 |
Diaz-Oviedo, Andrea I (12)
|
GUHCAP |
|
53.70 |
- |
|
Moultrie, Alden S (11)
|
GUTTST |
53.78 |
DQ |
- |
|
Moya, Megan L (12)
|
GUSHAC |
|
NS |
- |
|
Lorber, Lyla G (11)
|
GUESCA |
48.20 |
NS |
- |
|
Gomez, Abigail (12)
|
GUHCAP |
46.34 |
NS |
- |