| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ceraudo, Savannah G (10)
|
GUSHAC |
37.57 |
35.89 |
-1.68 |
| 2 |
Johnson, Cadence C (9)
|
GUSHAC |
38.24 |
35.90 |
-2.34 |
| 3 |
Voelker, Erin A (10)
|
GUAQUA |
39.01 |
37.44 |
-1.57 |
| 4 |
St John, Laura E (10)
|
GUSHAC |
37.39 |
38.39 |
1.00 |
| 5 |
Ceraudo, Olivia M (10)
|
GUSHAC |
39.93 |
38.80 |
-1.13 |
| 6 |
Elgar, Kate Jasmine E (10)
|
GUSHAC |
39.94 |
39.28 |
-0.66 |
| 7 |
Qiu, Annabel Y (9)
|
GU300 |
41.54 |
39.49 |
-2.05 |
| 7 |
Johnson, Addison E (10)
|
GUHCAP |
39.89 |
39.49 |
-0.40 |
| 9 |
Coulon, Myla S (10)
|
GUESCA |
38.62 |
40.45 |
1.83 |
| 10 |
Turan, Ecem Y (9)
|
GUESCA |
42.90 |
41.12 |
-1.78 |
| 11 |
Rodriguez, Bridget A (10)
|
GUHCAP |
|
43.03 |
- |
| 12 |
Coward, Kayin S (10)
|
GUHCAP |
|
44.38 |
- |
| 13 |
Herbst, Grace E (10)
|
GUSHAC |
44.21 |
45.52 |
1.31 |
| 14 |
Puente, Allison (10)
|
GUHCAP |
46.38 |
45.95 |
-0.43 |
| 15 |
Menifee, Mikinzi G (10)
|
GUHCAP |
|
46.55 |
- |
| 16 |
Nguyen, Nina T (9)
|
GUESCA |
46.86 |
47.27 |
0.41 |
| 17 |
Willemse, Tara A (9)
|
GUCOOG |
53.67 |
49.88 |
-3.79 |
| 18 |
Lee, Julianne Y (9)
|
GU300 |
|
50.62 |
- |
| 19 |
Sinha, Anisa J (9)
|
GUCOOG |
52.36 |
53.64 |
1.28 |
| 20 |
Lau, Eilene H (9)
|
GUSHAC |
50.38 |
53.67 |
3.29 |
| 21 |
Du, Danica (10)
|
GU300 |
|
55.40 |
- |
| 22 |
Marceleno, Valeria (9)
|
GUHCAP |
55.95 |
56.91 |
0.96 |
| 23 |
Singh, Tvisha (9)
|
GUECS |
|
59.00 |
- |
| 24 |
Herbst, Emily F (9)
|
GUSHAC |
52.97 |
1:00.25 |
7.28 |
| 25 |
Krishnan, Mihika H (9)
|
GU300 |
|
1:00.76 |
- |
| 26 |
Chelius, Naomi T (10)
|
GUCOOG |
1:00.42 |
1:05.41 |
4.99 |
|
Hinojo, Irais (10)
|
GUESCA |
46.86 |
NS |
- |
|
Obua, Cara O (9)
|
GUECS |
57.73 |
NS |
- |
|
Hernandez, Sophia V (9)
|
GUECS |
|
NS |
- |