| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Cadence C (9)
|
GUSHAC |
31.83 |
32.19 |
0.36 |
| 2 |
Ceraudo, Savannah G (10)
|
GUSHAC |
32.25 |
32.65 |
0.40 |
| 3 |
Ceraudo, Olivia M (10)
|
GUSHAC |
37.21 |
34.52 |
-2.69 |
| 4 |
Voelker, Erin A (10)
|
GUAQUA |
34.53 |
35.67 |
1.14 |
| 5 |
Morgan, Jayde K (9)
|
GUSHAC |
40.40 |
39.27 |
-1.13 |
| 6 |
Qiu, Annabel Y (9)
|
GU300 |
40.94 |
40.94 |
0.00 |
| 7 |
Elgar, Kate Jasmine E (10)
|
GUSHAC |
43.78 |
41.84 |
-1.94 |
| 8 |
Herbst, Grace E (10)
|
GUSHAC |
45.67 |
43.68 |
-1.99 |
| 9 |
Rodriguez, Bridget A (10)
|
GUHCAP |
|
44.48 |
- |
| 10 |
Lau, Eilene H (9)
|
GUSHAC |
51.19 |
50.15 |
-1.04 |
| 11 |
Willemse, Tara A (9)
|
GUCOOG |
56.00 |
51.96 |
-4.04 |
| 12 |
Sinha, Anisa J (9)
|
GUCOOG |
|
55.49 |
- |
| 13 |
Krishnan, Mihika H (9)
|
GU300 |
|
1:01.46 |
- |
|
Coward, Kayin S (10)
|
GUHCAP |
|
DQ |
- |
|
Zhao, Serena (9)
|
GU300 |
43.61 |
NS |
- |
|
Hinojo, Irais (10)
|
GUESCA |
43.48 |
NS |
- |
|
Hinojo, Alani (9)
|
GUESCA |
1:07.91 |
NS |
- |