| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tat, Thomas A (14)
|
GUSHAC |
24.18 |
24.55 |
0.37 |
| 2 |
Chin, Thomas Zhee Yang (14)
|
GUECS |
25.78 |
24.93 |
-0.85 |
| 3 |
Nuval, Mack A (13)
|
GUUN-S |
24.90 |
25.14 |
0.24 |
| 4 |
Hensley, David G (14)
|
GUSHRK |
25.75 |
25.58 |
-0.17 |
| 5 |
McDonald, Christopher R (14)
|
GUSHAC |
25.98 |
25.67 |
-0.31 |
| 6 |
Walker, William Jack (14)
|
GUECS |
27.51 |
26.01 |
-1.50 |
| 7 |
Arismendez-Reyes, Lorenzo I (13)
|
GUSHAC |
27.44 |
26.40 |
-1.04 |
| 8 |
Broughton, Jeremiah A (13)
|
GUHCAP |
26.23 |
26.59 |
0.36 |
| 9 |
Smith, Carson T (13)
|
GUSHAC |
26.55 |
26.86 |
0.31 |
| 10 |
Tran, Preston (13)
|
GUSHAC |
27.43 |
27.90 |
0.47 |
| 11 |
Larsson, Niklas (14)
|
GUECS |
30.84 |
29.20 |
-1.64 |
| 12 |
Hoffman, Cade C (13)
|
GUSHAC |
30.20 |
29.39 |
-0.81 |
| 13 |
Cao, Kyler A (13)
|
GUTTST |
33.26 |
29.54 |
-3.72 |
| 14 |
Blanco, Joshua A (14)
|
GUSHAC |
32.31 |
30.41 |
-1.90 |
| 15 |
Grabein, Aiden K (13)
|
GUSHAC |
36.22 |
35.57 |
-0.65 |