| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fuentes, Martina N (15)
|
GUSHAC |
54.86 |
55.26 |
0.40 |
| 2 |
Howard, Olivia R (15)
|
GUSHAC |
54.06 |
55.86 |
1.80 |
| 3 |
Niles, Kalissa D (15)
|
GUECS |
55.82 |
56.59 |
0.77 |
| 4 |
Kurka, Pearson E (15)
|
GUSHAC |
56.05 |
58.39 |
2.34 |
| 5 |
Nguyen, Breanna B (17)
|
GUTTST |
57.47 |
58.51 |
1.04 |
| 6 |
Bailey, Sierra R (15)
|
GUECS |
57.22 |
59.94 |
2.72 |
| 7 |
Dronova, Sophia V (15)
|
GUSHAC |
59.26 |
1:01.47 |
2.21 |
| 8 |
Hernandez, Kenya (15)
|
GUSHAC |
1:03.15 |
1:01.64 |
-1.51 |
| 9 |
Orellana, Natalie E (15)
|
GUHCAP |
58.36 |
1:01.79 |
3.43 |
| 10 |
Sarkari, Rukshana R (17)
|
GUSHAC |
58.98 |
1:01.91 |
2.93 |
| 11 |
Gaddis, Juliana P (15)
|
GUHCAP |
1:00.66 |
1:02.53 |
1.87 |
| 12 |
Renderos, Melanie D (16)
|
GUHCAP |
59.08 |
1:03.25 |
4.17 |
| 13 |
Zwerneman, Hannah N (18)
|
GUTTST |
1:02.21 |
1:04.97 |
2.76 |
| 14 |
Parker, Allie T (16)
|
GULAP |
1:05.27 |
1:05.21 |
-0.06 |
| 15 |
Samson, Camillia V (16)
|
GUTTST |
1:08.41 |
1:05.35 |
-3.06 |
| 16 |
Wachsler, Catherine N (17)
|
GULAP |
1:01.49 |
1:05.52 |
4.03 |
| 17 |
Castro, Johana J (15)
|
GUHCAP |
1:01.93 |
1:06.01 |
4.08 |
| 18 |
Johnson, Beatriz Andrea A (15)
|
GUECS |
1:10.49 |
1:09.00 |
-1.49 |