| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chien, Amber Y (12)
|
GUCFSC |
2:59.20 |
2:59.26 |
0.06 |
| 2 |
Shimel, Julia A (12)
|
GUESA |
2:58.32 |
3:03.35 |
5.03 |
| 3 |
Ianeva, Monica R (11)
|
GUTWST |
3:09.81 |
3:09.99 |
0.18 |
| 4 |
Obar, Lola M (11)
|
GUCFSC |
3:15.19 |
3:12.63 |
-2.56 |
| 5 |
Smith, Ashlee M (12)
|
GUCFSC |
3:13.32 |
3:16.83 |
3.51 |
| 6 |
De Amicis, Emily R (11)
|
GUECS |
3:18.39 |
3:18.13 |
-0.26 |
| 7 |
Fu, Angela (11)
|
STNTRO |
3:19.49 |
3:18.29 |
-1.20 |
| 8 |
Maddan, Erin F (11)
|
GUCFSC |
|
3:19.93 |
- |
| 9 |
Seal, Hannah M (12)
|
STNTRO |
3:16.10 |
3:21.10 |
5.00 |
| 10 |
Fulton, Elsa A (11)
|
GUTWST |
3:19.81 |
3:22.99 |
3.18 |
| 11 |
Wolfe, Meredith C (11)
|
STNTRO |
3:20.60 |
3:25.37 |
4.77 |
| 12 |
Shepherd, Gabriella F (12)
|
GUCFSC |
3:29.45 |
3:28.26 |
-1.19 |
| 13 |
Adair, Sydney E (12)
|
GUESA |
|
3:30.28 |
- |
| 14 |
Halverson, Allie C (12)
|
GUTWST |
3:14.81 |
3:30.82 |
16.01 |
| 15 |
White, Margaret V (11)
|
GUTWST |
3:29.81 |
3:32.98 |
3.17 |
| 16 |
Dai, Melinda M (11)
|
STNTRO |
3:25.41 |
3:33.15 |
7.74 |
| 17 |
Ivins, Natalie E (11)
|
GUSSAN |
3:21.73 |
3:39.70 |
17.97 |
| 18 |
Marascio, Ellington K (11)
|
GUTWST |
3:29.81 |
3:40.43 |
10.62 |
| 19 |
Mitchell, Haley A (11)
|
GUCFSC |
|
3:41.64 |
- |
| 20 |
Miller, Rachel B (12)
|
STNTRO |
3:31.99 |
3:42.72 |
10.73 |
| 21 |
Alter, Haven M (12)
|
GUSSAN |
3:52.05 |
3:52.02 |
-0.03 |