| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kumar, Isabella K (14)
|
GUFCST |
2:27.74 |
2:26.74 |
-1.00 |
| 2 |
Curran, Trinity E (13)
|
GUMAC |
2:31.63 |
2:28.72 |
-2.91 |
| 3 |
Boulaknadal, Yasmeen (13)
|
GUECS |
2:32.08 |
2:32.12 |
0.04 |
| 4 |
Rollinson, Kira A (14)
|
GUFCST |
2:39.72 |
2:32.91 |
-6.81 |
| 5 |
Ramos, Ava Lee B (13)
|
GUFCST |
2:35.13 |
2:33.85 |
-1.28 |
| 6 |
Jacob, Kimberly M (14)
|
GUFCST |
2:39.67 |
2:37.02 |
-2.65 |
| 7 |
Granda-Moncayo, Ariana V (13)
|
GUAGS |
2:43.79 |
2:39.69 |
-4.10 |
| 8 |
Smith, Colleen C (13)
|
GUMAC |
|
2:41.36 |
- |
| 9 |
Hyman, Zoe A (13)
|
GUAGS |
|
2:42.27 |
- |
| 10 |
Anderson, Anneke C (13)
|
GUMAC |
2:40.72 |
2:42.35 |
1.63 |
| 11 |
Ranjan, Khyatee (13)
|
GUFCST |
2:44.99 |
2:43.06 |
-1.93 |
| 12 |
Cunningham, Margaret G (13)
|
GUAGS |
2:40.37 |
2:43.61 |
3.24 |
| 13 |
Castaneda, Mariana Y (13)
|
GUTWST |
|
2:56.93 |
- |
| 14 |
Wesley, Alyssa B (13)
|
GUTWST |
|
3:00.11 |
- |
|
Gough, Cerys D (13)
|
GUECS |
2:28.35 |
DQ |
- |
|
Liau, Olivia P (13)
|
GUFCST |
|
NS |
- |
|
Harkrider, Haley L (14)
|
GUFCST |
2:52.40 |
NS |
- |
|
Grames, Josie K (13)
|
GUECS |
2:55.04 |
NS |
- |
|
Ali, Ruhayma A (13)
|
GUAGS |
|
NS |
- |