| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Garcia, Lauren G (14)
|
GUFCST |
2:52.78 |
2:49.75 |
-3.03 |
| 2 |
Smith, Colleen C (13)
|
GUMAC |
2:52.73 |
2:50.73 |
-2.00 |
| 3 |
Connell, Arina L (13)
|
GUFCST |
2:52.26 |
2:51.80 |
-0.46 |
| 4 |
Anderson, Anneke C (13)
|
GUMAC |
2:50.98 |
2:52.30 |
1.32 |
| 5 |
Kumar, Isabella K (14)
|
GUFCST |
2:59.44 |
3:02.72 |
3.28 |
| 6 |
Ho, Tonya K (13)
|
GUFCST |
3:03.57 |
3:04.40 |
0.83 |
| 7 |
Cunningham, Margaret G (13)
|
GUAGS |
3:11.56 |
3:04.48 |
-7.08 |
| 8 |
Sanchez, Kimberly (13)
|
GUMAC |
|
3:07.33 |
- |
| 9 |
Barnett, Araceli M (14)
|
GUTWST |
3:17.56 |
3:13.93 |
-3.63 |
| 10 |
Castaneda, Mariana Y (13)
|
GUTWST |
|
3:22.80 |
- |
| 11 |
Almazan Guerrero, Diana C (13)
|
GUMAC |
3:22.90 |
3:31.68 |
8.78 |
|
Guan, Yingqi (14)
|
GUAGS |
3:11.88 |
NS |
- |
|
Christman, Emily Y (13)
|
GUFCST |
3:18.16 |
NS |
- |
|
Vessel, Mixon N (13)
|
GUTWST |
|
NS |
- |