| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hembree, Ashlyn J (15)
|
GUFCST |
2:25.50 |
2:31.49 |
5.99 |
| 2 |
Rollinson, Keely C (16)
|
GUFCST |
2:26.28 |
2:37.35 |
11.07 |
| 3 |
Garriga, Isabella A (17)
|
GUFCST |
2:38.03 |
2:40.54 |
2.51 |
| 4 |
Brown, Meredith E (15)
|
GUFCST |
2:34.64 |
2:43.46 |
8.82 |
| 5 |
Kulow, Katelyn M (16)
|
GUFCST |
2:37.89 |
2:44.43 |
6.54 |
| 6 |
Hatrick, Lucy C (16)
|
GUTWST |
2:36.60 |
2:47.48 |
10.88 |
| 7 |
Ball, Karloyn E (17)
|
GUESA |
2:18.16 |
2:48.02 |
29.86 |
| 8 |
Quinn, Caitlyn A (16)
|
GUFCST |
2:45.05 |
2:49.51 |
4.46 |
| 9 |
Walters, Meagan M (15)
|
GUFCST |
2:56.22 |
2:51.75 |
-4.47 |
| 10 |
Zhao, Susan A (15)
|
GUFCST |
2:49.36 |
3:00.22 |
10.86 |
| 11 |
Hoffman, Mackenzie L (16)
|
GUSWIM |
3:09.16 |
3:03.06 |
-6.10 |
| 12 |
Rao, Ananya (15)
|
GUECS |
3:06.94 |
3:04.04 |
-2.90 |
| 13 |
Chatram, Maygha (15)
|
GUFCST |
3:00.96 |
3:04.86 |
3.90 |
| 14 |
Pidgirsky, Anna I (15)
|
GUFCST |
3:03.41 |
3:06.52 |
3.11 |
| 15 |
Johnson, Beatriz Andrea A (15)
|
GUECS |
3:12.00 |
3:09.18 |
-2.82 |
| 16 |
Vanderkooi, Rachel H (17)
|
GUTYES |
3:16.52 |
3:18.18 |
1.66 |