| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nace, Jackson E (14)
|
GUTYES |
34.73 |
35.91 |
1.18 |
| 2 |
Nendza, Joseph T (13)
|
GUSCAT |
43.55 |
39.95 |
-3.60 |
| 3 |
Radhakrishnan, Arjun (14)
|
GUTWST |
40.98 |
40.64 |
-0.34 |
| 4 |
Adesina, Fiyinfoluwa P (13)
|
GUECS |
41.10 |
40.92 |
-0.18 |
| 5 |
Khong, Justin T (13)
|
GUTWST |
51.99 |
44.09 |
-7.90 |
| 6 |
Deniz, Alp (14)
|
GUFCST |
49.28 |
44.70 |
-4.58 |
| 7 |
Strube, Clayton A (14)
|
GUESA |
42.72 |
45.98 |
3.26 |
| 8 |
Lucero, Adrian R (14)
|
GUTYES |
56.97 |
46.18 |
-10.79 |
| 9 |
Shah, Dhrishit A (13)
|
GUFCST |
48.65 |
48.65 |
0.00 |
| 10 |
Wang, Jin (13)
|
GUFCST |
45.18 |
50.02 |
4.84 |
| 11 |
Frismanis, Spencer C (13)
|
GUFCST |
55.46 |
51.51 |
-3.95 |
| 12 |
Johnson, Michael A (13)
|
GUFCST |
52.62 |
51.76 |
-0.86 |
| 13 |
Ralph, Noah A (13)
|
GUTWST |
1:01.14 |
52.18 |
-8.96 |
| 14 |
Nie, Delbert M (13)
|
GU300 |
54.42 |
53.93 |
-0.49 |
| 15 |
Rodriguez Oregon, Iker E (13)
|
GUTWST |
|
54.13 |
- |
| 16 |
Chilakalapudi, Mridul (13)
|
GUFCST |
52.69 |
54.72 |
2.03 |
| 17 |
Dharia, Rohan T (13)
|
GUFCST |
45.64 |
55.46 |
9.82 |
| 18 |
Pineda, Jose V (13)
|
GUESA |
55.97 |
1:01.56 |
5.59 |
| 19 |
Lau, Jason Y (14)
|
GUFCST |
58.69 |
1:02.36 |
3.67 |
|
Crist, Alexander W (13)
|
GUFCST |
48.49 |
DQ |
- |