| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Feely, Caitlyn E (12)
|
GUSWIM |
36.14 |
36.61 |
0.47 |
| 2 |
Ramsower, Ava M (11)
|
GUFCST |
37.14 |
37.18 |
0.04 |
| 3 |
Pan, Sophie A (12)
|
GUFCST |
39.08 |
38.66 |
-0.42 |
| 4 |
Duncan, Grace K (11)
|
GUESA |
34.97 |
38.70 |
3.73 |
| 5 |
Silva, Laura S (12)
|
GUTWST |
41.55 |
39.64 |
-1.91 |
| 6 |
Vazquez, Michelle (12)
|
GUESA |
37.08 |
42.23 |
5.15 |
| 7 |
Radford, Mackenzie M (11)
|
GUTWST |
44.06 |
42.78 |
-1.28 |
| 8 |
Gumban, Lyra A (11)
|
GUFCST |
44.25 |
43.01 |
-1.24 |
| 9 |
Mccormack, Ava C (12)
|
GUSST |
36.47 |
43.04 |
6.57 |
| 10 |
Lopez, Isabella M (12)
|
GUTWST |
45.87 |
44.09 |
-1.78 |
| 11 |
Gaudette, Sophie C (11)
|
GUFCST |
49.39 |
44.22 |
-5.17 |
| 12 |
Light, Ava S (12)
|
GUFCST |
44.99 |
44.25 |
-0.74 |
| 13 |
Gonzalez, Skylar S (11)
|
GUTWST |
42.25 |
44.49 |
2.24 |
| 14 |
Hadley, Kaylor N (11)
|
GUTYES |
45.42 |
46.31 |
0.89 |
| 15 |
Jayaraman, Elakiya A (12)
|
GUTWST |
1:01.60 |
47.38 |
-14.22 |
| 16 |
Vann, Eden E (11)
|
GUFCST |
54.82 |
50.53 |
-4.29 |
| 17 |
Lau, Janice Y (11)
|
GUFCST |
1:00.58 |
54.30 |
-6.28 |