| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hennig, Jordan E (18)
|
GUSWIM |
29.16 |
30.10 |
0.94 |
| 2 |
Lanigan, Caitlin E (15)
|
GUTWST |
32.65 |
31.23 |
-1.42 |
| 3 |
Harrop, Rebecca J (17)
|
GUTWST |
30.53 |
31.37 |
0.84 |
| 4 |
Kooyers, Taryn K (16)
|
GUSWIM |
32.28 |
31.68 |
-0.60 |
| 5 |
Saldana, Gabriela A (16)
|
GUNOCH |
32.05 |
31.98 |
-0.07 |
| 6 |
Fields, Karsten E (18)
|
GUTWST |
30.43 |
33.09 |
2.66 |
| 7 |
Rodriguez Diaz, Jimena (15)
|
GUTWST |
33.88 |
33.64 |
-0.24 |
| 8 |
Bailey, Sierra R (15)
|
GUECS |
32.63 |
33.71 |
1.08 |
| 9 |
Lacson, Lauren E (16)
|
GUTWST |
35.18 |
34.11 |
-1.07 |
| 10 |
Sharkowicz, Julia (15)
|
GUTWST |
53.24 |
34.25 |
-18.99 |
| 11 |
Guerrero, Katia A (17)
|
GUTWST |
33.25 |
35.92 |
2.67 |
| 12 |
Gonzales, Chloe N (15)
|
GUNEHA |
34.58 |
38.50 |
3.92 |
| 13 |
Findley, Lauren N (16)
|
GUVSST |
38.19 |
39.73 |
1.54 |
| 14 |
Rao, Ananya (15)
|
GUECS |
42.29 |
41.64 |
-0.65 |
| 15 |
McKneely, Shelly S (15)
|
GUVSST |
39.32 |
44.06 |
4.74 |
|
Budsberg, Addison E (15)
|
GUTWST |
39.96 |
DQ |
- |
|
Rodriguez, Kristen L (16)
|
GUNOCH |
1:35.69 |
DQ |
- |