| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Choi, Lennox (14)
|
GUECS |
29.44 |
29.99 |
0.55 |
| 2 |
Wylie, Bryson L (14)
|
GUSWIM |
35.02 |
34.25 |
-0.77 |
| 3 |
Medina, Sebastian E (14)
|
GUCATS |
34.87 |
34.45 |
-0.42 |
| 4 |
Brewer, Nathan C (14)
|
GUSWIM |
36.49 |
34.73 |
-1.76 |
| 5 |
Adesina, Fiyinfoluwa P (13)
|
GUECS |
33.40 |
35.27 |
1.87 |
| 6 |
Hopkins, Samuel M (14)
|
GUVSST |
39.03 |
35.68 |
-3.35 |
| 7 |
Radhakrishnan, Arjun (14)
|
GUTWST |
38.73 |
37.61 |
-1.12 |
| 8 |
St. Amant, Knox T (13)
|
GUVSST |
37.56 |
38.88 |
1.32 |
| 9 |
Cheng-Guajardo, Noah P (14)
|
GUNEHA |
48.04 |
39.78 |
-8.26 |
| 10 |
Ralph, Noah A (13)
|
GUTWST |
42.20 |
42.12 |
-0.08 |
| 11 |
Del Corral, Javier F (14)
|
GUTWST |
1:00.00 |
42.33 |
-17.67 |
| 12 |
Quickfall Villar, John D (13)
|
GUECS |
41.50 |
45.91 |
4.41 |
|
Prabhu, Matthew K (13)
|
GU300 |
44.91 |
DQ |
- |
|
Rodabaugh, Lauren T (14)
|
GUSWIM |
1:20.99 |
NS |
- |