| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Monson, Abigail K (14)
|
GUTWST |
2:31.30 |
2:39.53 |
8.23 |
| 2 |
Uranga, Maria J (13)
|
GUTWST |
2:30.25 |
2:40.94 |
10.69 |
| 3 |
Gibbs, Kinley E (13)
|
GUCATS |
2:42.05 |
2:41.03 |
-1.02 |
| 4 |
Niewenhous, Lily G (14)
|
GUTWST |
2:41.16 |
2:43.67 |
2.51 |
| 5 |
Dautrich, Corry M (13)
|
GUTWST |
2:38.18 |
2:45.54 |
7.36 |
| 6 |
White, Beatrix V (13)
|
GUTWST |
2:45.67 |
2:45.71 |
0.04 |
| 7 |
Holovaty, Madison M (13)
|
GUTWST |
2:45.98 |
2:52.04 |
6.06 |
| 8 |
Rodriguez, Soledad T (14)
|
GUTWST |
2:50.79 |
2:53.54 |
2.75 |
| 9 |
Brooks, Kennedy J (14)
|
GUECS |
2:53.31 |
2:54.97 |
1.66 |
| 10 |
Faulkner, Katherine A (14)
|
GUCATS |
2:49.46 |
2:55.26 |
5.80 |
| 11 |
Gough, Cerys D (14)
|
GUECS |
3:02.23 |
3:01.44 |
-0.79 |
| 12 |
Cole, Kaylee J (14)
|
GUVSST |
3:05.87 |
3:01.77 |
-4.10 |
| 13 |
Mills, Kendall G (14)
|
GUSWIM |
2:56.05 |
3:04.39 |
8.34 |
| 14 |
Boulaknadal, Yasmeen (13)
|
GUECS |
3:02.81 |
3:08.29 |
5.48 |
|
Yates, Khiara I (14)
|
GUTWST |
2:42.47 |
DQ |
- |