| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Everett, Ainsley E (17)
|
GUTWST |
2:20.72 |
2:31.95 |
11.23 |
| 2 |
Mills, Amy M (17)
|
GUCATS |
2:33.32 |
2:35.63 |
2.31 |
| 3 |
Hennig, Jordan E (18)
|
GUSWIM |
2:30.46 |
2:35.75 |
5.29 |
| 4 |
Kooyers, Taryn K (16)
|
GUSWIM |
2:35.79 |
2:41.06 |
5.27 |
| 5 |
Gordon, Mary-Ashlynne G (15)
|
GUCATS |
2:35.17 |
2:41.17 |
6.00 |
| 6 |
Lacson, Lauren E (16)
|
GUTWST |
2:30.16 |
2:42.23 |
12.07 |
| 7 |
Garriss, Olivia M (15)
|
GUTWST |
2:43.54 |
2:49.01 |
5.47 |
| 8 |
Harrison, Madison T (15)
|
GUNEHA |
2:36.10 |
2:57.16 |
21.06 |
| 9 |
Vander Neut, Reilly A (15)
|
GUSWIM |
2:54.44 |
3:05.92 |
11.48 |
| 10 |
Rao, Ananya (15)
|
GUECS |
3:01.97 |
3:06.94 |
4.97 |