| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Adesina, Fiyinfoluwa P (13)
|
GUECS |
41.09 |
41.10 |
0.01 |
| 2 |
Martinez, Jorge (14)
|
GUVSST |
55.47 |
43.84 |
-11.63 |
| 3 |
Hopkins, Samuel M (14)
|
GUVSST |
45.42 |
44.41 |
-1.01 |
| 4 |
Croxton, Joseph R (14)
|
GUTWST |
45.53 |
44.74 |
-0.79 |
| 5 |
Brewer, Nathan C (14)
|
GUSWIM |
45.16 |
47.85 |
2.69 |
| 6 |
Quickfall Villar, John D (13)
|
GUECS |
44.45 |
49.04 |
4.59 |
| 7 |
So, Maximus K (13)
|
GUSWIM |
54.41 |
51.44 |
-2.97 |
| 8 |
Prabhu, Matthew K (13)
|
GU300 |
49.15 |
52.10 |
2.95 |
| 9 |
Patel, Corin S (13)
|
GUSWIM |
51.73 |
52.49 |
0.76 |
|
Del Corral, Javier F (14)
|
GUTWST |
1:00.00 |
NS |
- |
|
Stone, Daniel B (14)
|
GUTWST |
52.08 |
NS |
- |